Coding
Part:BBa_K4175012
Designed by: Li Xianxiu, Zhang Wanying Group: iGEM22_ZJUintl-China (2022-09-25)
UAS-pSV40-CAR19-P2A-mCherry
This is a second-generation anti-CD19 chimeric antigen receptor (CAR) that comprises of a 4-1BB motif (BBa_K4175006) and a CD3ζ motif (BBa_K4175009). It is controlled by UAS-pSV40 promoter (BBa_K4040033), where UAS acts as a binding region for Gal4 while SV40 is a strong promoter. The anti-CD19 CAR is linked to the fluorescent protein, mCherry, by P2A self-cleavage peptide. This peptide will undergo ribosomal skipping upon translation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1388
Illegal NgoMIV site found at 1803 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 949
[edit]
Categories
Parameters
biology | Human |